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Journal
/
Genetics
/
位点导向
的φC31
介导的疟疾
按蚊
载体中的整合和盒式交换
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Site-Directed
φC31
-Mediated Integration and Cassette Exchange in
Anopheles
Vectors of Malaria
Please note that all translations are automatically generated.
Click here for the English version.
位点导向
的φC31
介导的疟疾
按蚊
载体中的整合和盒式交换
DOI:
10.3791/62146-v
•
09:38 min
•
February 02, 2021
•
Adriana Adolfi
2
,
Amy Lynd
,
Gareth J. Lycett
,
Anthony A. James
3
1
Vector Biology Department
,
Liverpool School of Tropical Medicine
,
2
Department of Microbiology & Molecular Genetics
,
University of California
,
3
Department of Molecular Biology & Biochemistry
,
University of California
Chapters
00:04
Introduction
00:46
Preparation of Plasmids for the Microinjection Mix
02:33
Microinjection of Embryos from an
Anopheles
Docking Line
03:38
Crossing and Screening of Transformed Individuals
06:45
Results: Validation of φC31 Single Integration and RMCE in
An. stephensi
Larvae
08:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何使用
φC31
系统在
按蚊
的基因组中实现位点导向的修饰。所描述的修饰包括在携带
attP
的对接线的基因组中转基因盒的整合和交换。
Tags
Site-directed Integration
AttB-mediated Integration
Cassette Exchange
Anopheles
Malaria
Transgenesis
Transgenic Mosquitoes
Embryo Microinjection
Marker Gene
Helper Plasmid
Integrase
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