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Journal / Genetics / Site-Directed φC31-Mediated Integration and…
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Genetics

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JoVE Journal Genetics
Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
 

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Article DOI: 10.3791/62146-v • 09:38 min • February 2nd, 2021
February 2nd, 2021 •
1,2, 1, 1, 2,3
1Vector Biology Department, Liverpool School of Tropical Medicine, 2Department of Microbiology & Molecular Genetics, University of California, 3Department of Molecular Biology & Biochemistry, University of California

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The protocol describes how to achieve site-directed modifications in the genome of Anopheles malaria mosquitoes using the φC31 system. Modifications described include both the integration and the exchange of transgenic cassettes in the genome of attP-bearing docking lines.

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Keywords: Site-directed Integration AttB-mediated Integration Cassette Exchange Anopheles Malaria Transgenesis Transgenic Mosquitoes Embryo Microinjection Marker Gene Helper Plasmid Integrase
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