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Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
JoVE Journal
Genetik
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JoVE Journal Genetik
Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

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09:38 min

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February 02, 2021

DOI:

10.3791/62146-v

DOI:
10.3791/62146-v

•

09:38 min
February 02, 2021

•

1 Views
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1Vector Biology Department,Liverpool School of Tropical Medicine, 2Department of Microbiology & Molecular Genetics,University of California, 3Department of Molecular Biology & Biochemistry,University of California

Summary

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The protocol describes how to achieve site-directed modifications in the genome of Anopheles malaria mosquitoes using the φC31 system. Modifications described include both the integration and the exchange of transgenic cassettes in the genome of attP-bearing docking lines.

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Cite this Article

Adolfi, A., Lynd, A., Lycett, G. J., James, A. A. Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria. J. Vis. Exp. (168), e62146, doi:10.3791/62146 (2021).

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