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Site-Directed φC31-gemedieerde integratie en cassette-uitwisseling in Anopheles Vectors of Malaria
JoVE Journal
Genetics
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JoVE Journal Genetics
Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
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Site-Directed φC31-gemedieerde integratie en cassette-uitwisseling in Anopheles Vectors of Malaria

Nederlands

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3,536 Views

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09:38 min

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February 02, 2021

DOI:

10.3791/62146-v

DOI:
10.3791/62146-v

•

09:38 min
February 02, 2021

•

1 Views
, , ,
1Vector Biology Department,Liverpool School of Tropical Medicine, 2Department of Microbiology & Molecular Genetics,University of California, 3Department of Molecular Biology & Biochemistry,University of California

Summary

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Het protocol beschrijft hoe locatiegerichte modificaties in het genoom van Anopheles malariamuggen kunnen worden bereikt met behulp van het φC31-systeem . De beschreven modificaties omvatten zowel de integratie als de uitwisseling van transgene cassettes in het genoom van attP-dragende koppelingslijnen.

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Cite this Article

Adolfi, A., Lynd, A., Lycett, G. J., James, A. A. Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria. J. Vis. Exp. (168), e62146, doi:10.3791/62146 (2021).

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