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Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
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Genetics
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JoVE Journal Genetics
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

English

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8,307 Views

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10:10 min

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May 28, 2017

DOI:

10.3791/55919-v

DOI:
10.3791/55919-v

•

10:10 min
May 28, 2017

•

2 Views
, , , , , , ,
1Center for Genome Research, Department of Life Sciences,University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development,Imagine Institute, 3Institute for Biomedical Technologies,CNR, 4Généthon, 5Sorbonne Paris Cité – Université Paris Descartes

Summary

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Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.

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Romano, O., Cifola, I., Poletti, V., Severgnini, M., Peano, C., De Bellis, G., Mavilio, F., Miccio, A. Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions. J. Vis. Exp. (123), e55919, doi:10.3791/55919 (2017).

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