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Frazionamento cellulare di cellule U937 mediante purificazione del gradiente di densità isopicnica
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Biochemistry
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JoVE Journal Biochemistry
Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
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Frazionamento cellulare di cellule U937 mediante purificazione del gradiente di densità isopicnica

italiano

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3,337 Views

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10:19 min

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August 12, 2021

DOI:

10.3791/62119-v

DOI:
10.3791/62119-v

•

10:19 min
August 12, 2021

•

13 Views
, , , ,
1Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Summary

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Questo protocollo di frazionamento consentirà ai ricercatori di isolare proteine citoplasmatiche, nucleari, mitocondriali e di membrana da cellule di mammifero. Le ultime due frazioni subcellulari vengono ulteriormente purificate tramite gradiente di densità isopicnica.

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Cite this Article

McCaig, W. D., Deragon, M. A., Truong, P. V., Knapp, A. R., LaRocca, T. J. Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification. J. Vis. Exp. (174), e62119, doi:10.3791/62119 (2021).

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