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Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
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Biochemistry
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JoVE Journal Biochemistry
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

3,538 Views

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05:13 min

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June 30, 2021

DOI:

10.3791/62621-v

DOI:
10.3791/62621-v

•

05:13 min
June 30, 2021

•

13 Views
, ,
1School of Life Sciences,The Chinese University of Hong Kong

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Density Gradient UltracentrifugationEndocytic RecyclingMammalian CellsProtein TraffickingRecycling EndosomesCell FractionationSucrose GradientPost-nuclear SupernatantHomogenization BufferDounce HomogenizerProtease InhibitorPhosphatase Inhibitor

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Chan, W. W. R., Zhai, Y. Q., Lau, K. Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells. J. Vis. Exp. (172), e62621, doi:10.3791/62621 (2021).

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