Journal
/
Biochemistry
/
Polysome Profiling without Gradient Makers or Fractionation Systems
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Polysome Profiling without Gradient Makers or Fractionation Systems

Polysome Profiling without Gradient Makers or Fractionation Systems

DOI:
10.3791/62680-v

•

05:56 min

•

June 01, 2021

•

,
1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Chapters

  • 00:04Introduction
  • 00:39Preparation of 7 to 47% Sucrose Gradients
  • 02:14Preparation of Yeast Cell Extracts
  • 03:21Centrifugation of Gradients
  • 03:49Fraction and Data Collection
  • 04:36Results: Polysome Profiling Without Gradient Makers or Fractionation Systems
  • 05:19Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to generate a polysome profile without using automated gradient makers or gradient fractionation systems.

Tags

Polysome ProfilingGradient-freeSucrose GradientRibosome ActivityYeast Cell LysisRNA ExtractionRNA Quantification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Read Article