Journal
/
Biochemistry
/
Celfractionering van U937-cellen door isopycnic density gradient purification
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
Please note that all translations are automatically generated. Click here for the English version.

Celfractionering van U937-cellen door isopycnic density gradient purification

Nederlands

Automatically Generated

3,284 Views

•

10:19 min

•

August 12, 2021

DOI:

10.3791/62119-v

DOI:
10.3791/62119-v

•

10:19 min
August 12, 2021

•

13 Views
, , , ,
1Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Summary

Automatically generated

Dit fractioneringsprotocol stelt onderzoekers in staat om cytoplasmatische, nucleaire, mitochondriale en membraaneiwitten uit zoogdiercellen te isoleren. De laatste twee subcellulaire fracties worden verder gezuiverd via isopycnic dichtheidsgradiënt.

Explore More Videos

Cell FractionationU937 CellsIsopycnic Density Gradient PurificationCytoplasmNucleusMitochondriaMembraneCytosolic Protein IsolationNuclear Protein ExtractionCellular CompartmentsCell LysisCell HomogenizationDensity Gradient Centrifugation

Read Article

Cite this Article

McCaig, W. D., Deragon, M. A., Truong, P. V., Knapp, A. R., LaRocca, T. J. Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification. J. Vis. Exp. (174), e62119, doi:10.3791/62119 (2021).

Download .ris file

Copy

Share Video

.

Copy