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Celfractionering van U937-cellen door isopycnic density gradient purification
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Biochemistry
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JoVE Journal Biochemistry
Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
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Celfractionering van U937-cellen door isopycnic density gradient purification

Nederlands

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3,337 Views

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10:19 min

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August 12, 2021

DOI:

10.3791/62119-v

DOI:
10.3791/62119-v

•

10:19 min
August 12, 2021

•

13 Views
, , , ,
1Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Summary

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Dit fractioneringsprotocol stelt onderzoekers in staat om cytoplasmatische, nucleaire, mitochondriale en membraaneiwitten uit zoogdiercellen te isoleren. De laatste twee subcellulaire fracties worden verder gezuiverd via isopycnic dichtheidsgradiënt.

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Cite this Article

McCaig, W. D., Deragon, M. A., Truong, P. V., Knapp, A. R., LaRocca, T. J. Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification. J. Vis. Exp. (174), e62119, doi:10.3791/62119 (2021).

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