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Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
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August 12, 2021
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This fractionation protocol will allow researchers to isolate cytoplasmic, nuclear, mitochondrial, and membrane proteins from mammalian cells. The latter two subcellular fractions are further purified via isopycnic density gradient.
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McCaig, W. D., Deragon, M. A., Truong, P. V., Knapp, A. R., LaRocca, T. J. Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification. J. Vis. Exp. (174), e62119, doi:10.3791/62119 (2021).
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