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通过等密度梯度纯化对U937细胞进行细胞分级分离
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Biochemistry
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JoVE Journal Biochemistry
Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
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通过等密度梯度纯化对U937细胞进行细胞分级分离

3,239 Views

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10:19 min

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August 12, 2021

DOI:

10.3791/62119-v

DOI:
10.3791/62119-v

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10:19 min
August 12, 2021

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10 Views
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1Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Tags

Cell FractionationU937 CellsIsopycnic Density Gradient PurificationCytoplasmNucleusMitochondriaMembraneCytosolic Protein IsolationNuclear Protein ExtractionCellular CompartmentsCell LysisCell HomogenizationDensity Gradient Centrifugation

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Cite this Article

McCaig, W. D., Deragon, M. A., Truong, P. V., Knapp, A. R., LaRocca, T. J. Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification. J. Vis. Exp. (174), e62119, doi:10.3791/62119 (2021).

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