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Developmental Biology
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细胞细胞成像的微管细胞和微机械操作的
阿拉比多普西斯拍摄
阿皮克·梅里斯特姆
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the
Arabidopsis
Shoot Apical Meristem
Please note that all translations are automatically generated.
Click here for the English version.
细胞细胞成像的微管细胞和微机械操作的
阿拉比多普西斯拍摄
阿皮克·梅里斯特姆
DOI:
10.3791/60936-v
•
07:52 min
•
May 23, 2020
•
Yang Wang
,
Arun Sampathkumar
1
Max Planck Institute of Molecular Plant Physiology
Chapters
00:04
Introduction
00:34
Plant Growth
01:18
Shoot Apical Meristem (SAM) Dissection
02:14
Cultured SAM Transfer and Growth
02:57
SAM Imaging
04:57
Micromechanical SAM Perturbation and Data Analysis
06:23
Results: Representative Cortical Microtubule Anisotropy Quantification and Mechanical Ablation in Arabidopsis SAM
07:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个协议,用于在拍摄时皮质微管细胞骨架的活细胞成像,并监测其对物理力变化的反应。
Tags
Microtubule Cytoskeleton
Live Cell Imaging
Arabidopsis
Shoot Apical Meristem
Mechanical Manipulation
Plant Growth
Plant Development
Dissection
Microscopy
Plant Genetics
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