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Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

English

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11,314 Views

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10:03 min

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August 01, 2017

DOI:

10.3791/55914-v

DOI:
10.3791/55914-v

•

10:03 min
August 01, 2017

•

2 Views
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1Haematopoietic Stem Cell Laboratory,The Francis Crick Institute

Summary

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A method to create and live-image different humanized bone-marrow niches in mice is presented. Based on the supportive niche created by human mesenchymal cells, the addition of human endothelial cells induces the formation of human vessels, while the addition of rhBMP-2 induces the formation of human-mouse chimeric mature bone tissue.

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Passaro, D., Abarrategi, A., Foster, K., Ariza-McNaughton, L., Bonnet, D. Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging. J. Vis. Exp. (126), e55914, doi:10.3791/55914 (2017).

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