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Live Confocal Imaging of Developing Arabidopsis Flowers
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Live Confocal Imaging of Developing Arabidopsis Flowers

Live Confocal Imaging of Developing Arabidopsis Flowers

DOI:
10.3791/55156-v

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07:27 min

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April 01, 2017

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1Department of Biology and Biological Engineering,California Institute of Technology

Chapters

  • 00:05Title
  • 00:39Shoot Apex Dissection
  • 02:13Staining, Imaging Setup, and Sepal Ablation
  • 05:28Results: Representative Confocal Z-stacks of Live Flower Buds
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

Live confocal imaging provides biologists with a powerful tool to study development. Here, we present a detailed protocol for the live confocal imaging of developing Arabidopsis flowers.

Tags

Live Confocal ImagingArabidopsis FlowersFlower DevelopmentFlower Organ FormationFlower Stem CellsDissecting InflorescenceAgaroseSterile Deionized WaterStage 7 Flower BudsImaging DishStainingInverted Microscope

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