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Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

DOI:
10.3791/60936-v

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07:52 min

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May 23, 2020

•

,
1Max Planck Institute of Molecular Plant Physiology

Chapters

  • 00:04Introduction
  • 00:34Plant Growth
  • 01:18Shoot Apical Meristem (SAM) Dissection
  • 02:14Cultured SAM Transfer and Growth
  • 02:57SAM Imaging
  • 04:57Micromechanical SAM Perturbation and Data Analysis
  • 06:23Results: Representative Cortical Microtubule Anisotropy Quantification and Mechanical Ablation in Arabidopsis SAM
  • 07:15Conclusion

Summary

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自动翻译

Here we describe a protocol for live cell imaging of the cortical microtubule cytoskeleton at the shoot apical meristem and monitoring its response to changes in physical forces.

Tags

Microtubule CytoskeletonLive Cell ImagingArabidopsisShoot Apical MeristemMechanical ManipulationPlant GrowthPlant DevelopmentDissectionMicroscopyPlant Genetics

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