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Live-imaging of the Drosophila Pupal Eye
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Live-imaging of the Drosophila Pupal Eye

Live-imaging of the Drosophila Pupal Eye

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09:54 min

January 12, 2015

DOI:

09:54 min
January 12, 2015

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Summary

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This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.

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