Journal
/
Developmental Biology
/
Live Cell Imaging of Chromosome Segregation During Mitosis
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

English

Automatically Generated

9,086 Views

•

06:39 min

•

March 14, 2018

DOI:

10.3791/57389-v

DOI:
10.3791/57389-v

•

06:39 min
March 14, 2018

•

7 Views
, ,
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

Summary

Automatically generated

This protocol describes an easy and convenient method to label and visualize live chromosomes in mitotic cells using Histone2B-GFP BacMam 2.0 label and a spinning disc confocal microscopy system.

Explore More Videos

Live Cell ImagingChromosome SegregationMitosisHistone 2B-GFP BacMam 2.0Spinning Disc Confocal MicroscopyMouse Embryonic FibroblastsNocodazoleCell SynchronizationLive Chromosome VisualizationEnvironmental ChamberOil Immersion Objective

Read Article

Cite this Article

Varadkar, P., Takeda, K., McCright, B. Live Cell Imaging of Chromosome Segregation During Mitosis. J. Vis. Exp. (133), e57389, doi:10.3791/57389 (2018).

Download .ris file

Copy

Share Video

.

Copy