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Laser Capture Mikrodissektion von Maus embryonalen Knorpel und Knochen für Die Genexpressionsanalyse
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Developmental Biology
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JoVE Journal Developmental Biology
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
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Laser Capture Mikrodissektion von Maus embryonalen Knorpel und Knochen für Die Genexpressionsanalyse

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6,882 Views

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09:20 min

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December 18, 2019

DOI:

10.3791/60503-v

DOI:
10.3791/60503-v

•

09:20 min
December 18, 2019

•

3 Views
, , , ,
1Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology,Icahn School of Medicine at Mount Sinai

Summary

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Dieses Protokoll beschreibt lasercapture microdissection for the isolation of knorpel and bone from fresh frozen sections of the mouse embryo. Knorpel und Knochen können durch Kresylviolettfärbung schnell visualisiert und präzise gesammelt werden, um hochwertige RNA für die transkriptomische Analyse zu liefern.

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Cite this Article

Wu, M., Kriti, D., van Bakel, H., Jabs, E. W., Holmes, G. Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis. J. Vis. Exp. (154), e60503, doi:10.3791/60503 (2019).

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