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Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
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Developmental Biology
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JoVE Journal Developmental Biology
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

English

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12,056 Views

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10:03 min

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May 10, 2019

DOI:

10.3791/59113-v

DOI:
10.3791/59113-v

•

10:03 min
May 10, 2019

•

7 Views
, ,
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology,Wuhan University, 2Department of Biologic and Materials Sciences, School of Dentistry,University of Michigan

Summary

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Here, we present a detailed protocol to detect and quantify protein levels during craniofacial morphogenesis/pathogenesis by immunostaining using mouse craniofacial tissues as examples. In addition, we describe a method for preparation and cryosectioning of undecalcified hard tissues from young mice for immunostaining.

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Cite this Article

Yang, J., Pan, H., Mishina, Y. Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone. J. Vis. Exp. (147), e59113, doi:10.3791/59113 (2019).

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