Journal
/
发育生物学
/
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

6,882 Views

•

09:20 min

•

December 18, 2019

DOI:

10.3791/60503-v

DOI:
10.3791/60503-v

•

09:20 min
December 18, 2019

•

3 Views
, , , ,
1Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology,Icahn School of Medicine at Mount Sinai

Summary

Automatically generated

This protocol describes laser capture microdissection for the isolation of cartilage and bone from fresh frozen sections of the mouse embryo. Cartilage and bone can be rapidly visualized by cresyl violet staining and collected precisely to yield high quality RNA for transcriptomic analysis.

Explore More Videos

Laser Capture MicrodissectionMouse Embryonic CartilageMouse Embryonic BoneGene Expression AnalysisCresyl Violet StainingRNA IntegrityCryosectioningOptimal Cutting Temperature CompoundPEN Membrane SlidesEthanolXylene

Read Article

Cite this Article

Wu, M., Kriti, D., van Bakel, H., Jabs, E. W., Holmes, G. Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis. J. Vis. Exp. (154), e60503, doi:10.3791/60503 (2019).

Download .ris file

Copy

Share Video

.

Copy