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用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分
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Developmental Biology
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JoVE Journal Developmental Biology
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
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用于基因表达分析的小鼠胚胎软骨和骨骼的激光捕获微分

DOI:
10.3791/60503-v

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09:20 min

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December 18, 2019

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, , , ,
1Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:04Title
  • 01:04Preparation of Fresh Frozen Specimen
  • 01:28Cryosectioning for Laser Capture Microdissection
  • 02:45Sample Preparation for LCM
  • 04:14Laser Capture Microdissection
  • 06:25Lysis of Microdissected Tissues and RNA Isolation
  • 06:54Results: Analysis of Laser Capture Microdissected Tissue
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了激光捕获微解剖，用于从小鼠胚胎的新鲜冷冻部分分离软骨和骨骼。软骨和骨骼可以通过紫罗兰染色快速可视化，并精确收集，产生高质量的RNA进行转录分析。

Tags

Laser Capture MicrodissectionMouse Embryonic CartilageMouse Embryonic BoneGene Expression AnalysisCresyl Violet StainingRNA IntegrityCryosectioningOptimal Cutting Temperature CompoundPEN Membrane SlidesEthanolXylene

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