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Analisi della camera cardiaca sviluppo durante l'embriogenesi del Mouse utilizzando l'intero Monte epifluorescenza
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Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
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Analisi della camera cardiaca sviluppo durante l'embriogenesi del Mouse utilizzando l'intero Monte epifluorescenza

italiano

Automatically Generated

7,296 Views

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06:27 min

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April 17, 2019

DOI:

10.3791/59413-v

DOI:
10.3791/59413-v

•

06:27 min
April 17, 2019

•

9 Views
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1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Summary

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Vi presentiamo i protocolli per esaminare lo sviluppo del cuore del mouse usando la microscopia intero Monte epifluorescente su embrioni di topo sezionati da ventricolare specifico MLC-2v-tdTomato reporter topi knock-in. Questo metodo permette di visualizzare direttamente ogni fase della formazione ventricolare durante lo sviluppo del cuore del mouse senza laboriosi metodi istochimici.

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Cite this Article

Zhang, Z., Nam, Y. Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence. J. Vis. Exp. (146), e59413, doi:10.3791/59413 (2019).

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