Journal
/
Developmental Biology
/
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

English

Automatically Generated

7,295 Views

•

06:27 min

•

April 17, 2019

DOI:

10.3791/59413-v

DOI:
10.3791/59413-v

•

06:27 min
April 17, 2019

•

9 Views
,
1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Summary

Automatically generated

We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.

Explore More Videos

Cardiac Chamber DevelopmentMouse EmbryogenesisWhole Mount EpifluorescenceMLC-2v-tdTomatoHeart Tube FormationLoopingChamber FormationFluorescent ReporterDissectionGenotypingEpifluorescent Imaging

Read Article

Cite this Article

Zhang, Z., Nam, Y. Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence. J. Vis. Exp. (146), e59413, doi:10.3791/59413 (2019).

Download .ris file

Copy

Share Video

.

Copy