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Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
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Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

7,296 Views

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06:27 min

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April 17, 2019

DOI:

10.3791/59413-v

DOI:
10.3791/59413-v

•

06:27 min
April 17, 2019

•

9 Views
,
1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Summary

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We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.

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Cardiac Chamber DevelopmentMouse EmbryogenesisWhole Mount EpifluorescenceMLC-2v-tdTomatoHeart Tube FormationLoopingChamber FormationFluorescent ReporterDissectionGenotypingEpifluorescent Imaging

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Cite this Article

Zhang, Z., Nam, Y. Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence. J. Vis. Exp. (146), e59413, doi:10.3791/59413 (2019).

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