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Developmental Biology

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JoVE Journal Developmental Biology
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
 

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Article DOI: 10.3791/59413-v • 06:27 min • April 17th, 2019
April 17th, 2019 •
1,2,3, 1,2,3
1Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology, Vanderbilt University

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We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.

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Cardiac Chamber Development Mouse Embryogenesis Whole Mount Epifluorescence Heart Tube Formation Looping Full Chamber Formation MLC-2v-tdTomato Mice Fluorescent Reporter Mouse Lines Simple Method Regular Lab Setting Mating Female With Male Mice Vaginal Plugs Pregnant Dams Euthanized Abdominal Cavity Incision Uterine Horns Oviducts 10 Centimeter Petri Dish Ice Cold PBS Amniotic Sac Separation
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