Rivista
/
Biologia dello sviluppo
/
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

DOI:
10.3791/59413-v

•

06:27 min

•

April 17, 2019

•

,
1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Capitoli

  • 00:04Titolo
  • 00:45Mouse Embryo Collection and Dissection
  • 02:39Whole-mount Epifluorescence Imaging
  • 03:23Genotyping
  • 04:34Results: Cardiac Chamber Development During Mouse Embryogenesis in MLC-2v-tdTomato Knock-in Mice
  • 06:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.

Tags

Keywords: Cardiac Chamber DevelopmentMouse EmbryogenesisWhole Mount EpifluorescenceMLC-2v-tdTomatoHeart Tube FormationLoopingChamber FormationFluorescent ReporterDissectionGenotypingEpifluorescent Imaging

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/59413?article_type=v

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development
check_url/it/59413?article_type=v

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
check_url/it/59413?article_type=v

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
check_url/it/59413?article_type=v

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
check_url/it/59413?article_type=v

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
check_url/it/59413?article_type=v

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
check_url/it/59413?article_type=v

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues
check_url/it/59413?article_type=v

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
check_url/it/59413?article_type=v

Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis
check_url/it/59413?article_type=v

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Read Article