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Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

7,296 Views

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06:27 min

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April 17, 2019

DOI:

10.3791/59413-v

DOI:
10.3791/59413-v

•

06:27 min
April 17, 2019

•

9 Views
,
1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Summary

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We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.

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Cite this Article

Zhang, Z., Nam, Y. Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence. J. Vis. Exp. (146), e59413, doi:10.3791/59413 (2019).

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