/
/
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
A subscription to JoVE is required to view this content. Sign in or start your free trial.
9,385 Views
•
09:42 min
•
October 12, 2017
DOI:
1Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, 4Microscopy Core,Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience,Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute,Icahn School of Medicine at Mount Sinai
Summary
Automatically generated
Here, we describe a protocol for whole mount immunofluorescence and image-based quantitative volumetric analysis of early stage mouse embryos. We present this technique as a powerful approach to qualitatively and quantitatively assess cardiac structures during development, and propose that it may be widely adaptable to other organ systems.
Read Article
Cite this Article
Bardot, E., Tzavaras, N., Benson, D. L., Dubois, N. C. Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos. J. Vis. Exp. (128), e56446, doi:10.3791/56446 (2017).
Copy