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ATAC-seq test ADN Mitochondrial faible contamination de primaires humaines CD4 + Lymphocytes T
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Genetics
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JoVE Journal Genetics
ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
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ATAC-seq test ADN Mitochondrial faible contamination de primaires humaines CD4 + Lymphocytes T

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12,315 Views

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08:36 min

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March 22, 2019

DOI:

10.3791/59120-v

DOI:
10.3791/59120-v

•

08:36 min
March 22, 2019

•

9 Views
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1Department of Biology,Boston University, 2Department of Computer Science and Engineering,University of California San Diego, 3Bioinformatics Program,Boston University

Summary

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Nous présentons ici un protocole pour effectuer un test pour la transposase accessible chromatine séquençage (ATAC-seq) sur activé CD4 + lymphocytes humains. Le protocole a été modifié pour réduire au minimum la contamination mitochondrial DNA lectures de 50 % à 3 % grâce à l’introduction d’une nouvelle mémoire tampon de lyse.

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Cite this Article

Rickner, H. D., Niu, S., Cheng, C. S. ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes. J. Vis. Exp. (145), e59120, doi:10.3791/59120 (2019).

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