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ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
Capítulos
- 00:04Título
- 00:55Activation and Purification of CD4+ T Cells
- 04:36Assay for Transposase-accessible Chromatin Sequencing (ATAC-seq)
- 06:33Results: ATAC-seq and Sequencing Result Analysis
- 07:48Conclusion
Here, we present a protocol to perform an assay for transposase-accessible chromatin sequencing (ATAC-seq) on activated CD4+ human lymphocytes. The protocol has been modified to minimize contaminating mitochondrial DNA reads from 50% to 3% through the introduction of a new lysis buffer.
