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Genetics

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JoVE Journal Genetics
ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
 

ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Here, we present a protocol to perform an assay for transposase-accessible chromatin sequencing (ATAC-seq) on activated CD4+ human lymphocytes. The protocol has been modified to minimize contaminating mitochondrial DNA reads from 50% to 3% through the introduction of a new lysis buffer.

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ATAC-seq Low Mitochondrial DNA Contamination Primary Human CD4+ T Lymphocytes Lysis Buffer Contaminating Mitochondrial Reads Sequencing Costs Decrease Validated ATAC-seq Protocol Human Primary PBMCs Human Primary Monocytes Mouse Dendritic Cells Melanoma Cell Lines Wide Range Of Cell Types Sample Loss Minimization High-quality Results Small Numbers Of Cells Laboratory Equipment CD4+ T Cells Isolation RPMI Cell Suspension Density
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