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Manipulation of Gene Function in Mexican Cavefish
JoVE Journal
Genetics
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JoVE Journal Genetics
Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

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07:01 min

April 22, 2019

DOI:

07:01 min
April 22, 2019

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Summary

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We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

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