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Manipulation of Gene Function in Mexican Cavefish
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Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

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07:01 min

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April 22, 2019

DOI:

10.3791/59093-v

DOI:
10.3791/59093-v

•

07:01 min
April 22, 2019

•

8 Views
, , , , ,
1Department of Biological Sciences,Florida Atlantic University, 2Jupiter Life Science Initiative,Florida Atlantic University, 3Harriet L. Wilkes Honors College,Florida Atlantic University

Summary

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We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

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Cave FishAstyanax MexicanusGene Function ManipulationMorpholinoCRISPRTol2 TransposaseGenetic ToolsBiología del desarrolloModel Organism

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Stahl, B. A., Jaggard, J. B., Chin, J. S., Kowalko, J. E., Keene, A. C., Duboué, E. R. Manipulation of Gene Function in Mexican Cavefish. J. Vis. Exp. (146), e59093, doi:10.3791/59093 (2019).

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