Journal
/
Genética
/
Manipulation of Gene Function in Mexican Cavefish
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

DOI:
10.3791/59093-v

•

07:01 min

•

April 22, 2019

•

, , , , ,
1Department of Biological Sciences,Florida Atlantic University, 2Jupiter Life Science Initiative,Florida Atlantic University, 3Harriet L. Wilkes Honors College,Florida Atlantic University

Capítulos

  • 00:04Título
  • 00:47Tool Preparation and Single-Cell Stage Egg Collection
  • 01:34Pico-Injection
  • 03:36Screening of Morpholino-Injected Individuals, CRISPR Mutagenesis, and Tol2 Transmission
  • 04:35Results: Representative Post-Pico-Injection Functional and Phenotypical Analyses
  • 06:27Conclusion

Summary

Traducción Automática

Traducción Automática

We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

Tags

Cave FishAstyanax MexicanusGene Function ManipulationMorpholinoCRISPRTol2 TransposaseGenetic ToolsBiología del desarrolloModel Organism

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Manipulation of Ploidy in Caenorhabditis elegans

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Culturing and Manipulation of O9-1 Neural Crest Cells

Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Read Article