Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Manipulation of Gene Function in Mexican Cavefish
Chapitres
- 00:04Titre
- 00:47Tool Preparation and Single-Cell Stage Egg Collection
- 01:34Pico-Injection
- 03:36Screening of Morpholino-Injected Individuals, CRISPR Mutagenesis, and Tol2 Transmission
- 04:35Results: Representative Post-Pico-Injection Functional and Phenotypical Analyses
- 06:27Conclusion
We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.
