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Manipulation of Gene Function in Mexican Cavefish
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JoVE Journal Genetik
Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

8,722 Views

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07:01 min

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April 22, 2019

DOI:

10.3791/59093-v

DOI:
10.3791/59093-v

•

07:01 min
April 22, 2019

•

8 Views
, , , , ,
1Department of Biological Sciences,Florida Atlantic University, 2Jupiter Life Science Initiative,Florida Atlantic University, 3Harriet L. Wilkes Honors College,Florida Atlantic University

Summary

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We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

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Cave FishAstyanax MexicanusGene Function ManipulationMorpholinoCRISPRTol2 TransposaseGenetic ToolsEntwicklungsbiologieModel Organism

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Cite this Article

Stahl, B. A., Jaggard, J. B., Chin, J. S., Kowalko, J. E., Keene, A. C., Duboué, E. R. Manipulation of Gene Function in Mexican Cavefish. J. Vis. Exp. (146), e59093, doi:10.3791/59093 (2019).

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