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Manipulation of Gene Function in Mexican Cavefish
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Genetica
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JoVE Journal Genetica
Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

DOI:
10.3791/59093-v

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07:01 min

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April 22, 2019

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, , , , ,
1Department of Biological Sciences,Florida Atlantic University, 2Jupiter Life Science Initiative,Florida Atlantic University, 3Harriet L. Wilkes Honors College,Florida Atlantic University

Capitoli

  • 00:04Titolo
  • 00:47Tool Preparation and Single-Cell Stage Egg Collection
  • 01:34Pico-Injection
  • 03:36Screening of Morpholino-Injected Individuals, CRISPR Mutagenesis, and Tol2 Transmission
  • 04:35Results: Representative Post-Pico-Injection Functional and Phenotypical Analyses
  • 06:27Conclusion

Summary

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Traduzione automatica

We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

Tags

Cave FishAstyanax MexicanusGene Function ManipulationMorpholinoCRISPRTol2 TransposaseGenetic ToolsBiologia dello sviluppoModel Organism

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