ジャーナル
/
/
Manipulation of Gene Function in Mexican Cavefish
JoVE Journal
遺伝学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 遺伝学
Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

8,722 Views

07:01 min

April 22, 2019

DOI:

07:01 min
April 22, 2019

8 Views
, , , , ,

概要

Automatically generated

We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

Read Article