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ヒト多能性幹細胞の肝細胞への効率的な分化
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
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ヒト多能性幹細胞の肝細胞への効率的な分化

日本語

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8,213 Views

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07:37 min

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June 11, 2019

DOI:

10.3791/58975-v

DOI:
10.3791/58975-v

•

07:37 min
June 11, 2019

•

9 Views
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1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, 2Department of Developmental Biology,Stanford University School of Medicine

Summary

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このプロトコルは、ヒト多能性幹細胞(hPSC)から肝細胞様細胞を18日間で効率的に生成する単層、血清フリー法を詳細に述べている。これは、hPSCが肝細胞様細胞を形成する前に、原始的な筋、決定的な内因性内因性、後部前腸および肝芽前駆体のような中間細胞型に順次分化するように6つのステップを伴う。

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Cite this Article

Loh, K. M., Palaria, A., Ang, L. T. Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells. J. Vis. Exp. (148), e58975, doi:10.3791/58975 (2019).

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