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Effiziente Differenzierung menschlicher pluripotenter Stammzellen in Leberzellen
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
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Effiziente Differenzierung menschlicher pluripotenter Stammzellen in Leberzellen

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8,213 Views

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07:37 min

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June 11, 2019

DOI:

10.3791/58975-v

DOI:
10.3791/58975-v

•

07:37 min
June 11, 2019

•

9 Views
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1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, 2Department of Developmental Biology,Stanford University School of Medicine

Summary

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Dieses Protokoll beschreibt eine monolayer, serumfreie Methode zur effizienten Erzeugung von Hepatozyten-ähnlichen Zellen aus menschlichen pluripotenten Stammzellen (hPSCs) in 18 Tagen. Dies beinhaltet sechs Schritte, da hPSCs sequenziell in zwischengeschaltete Zelltypen wie den Primitivstreifen, das definitive Endoderm, das hintere Vorgut und die Leberknospenvorläufer differenzieren, bevor hepatozytenähnliche Zellen gebildet werden.

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Loh, K. M., Palaria, A., Ang, L. T. Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells. J. Vis. Exp. (148), e58975, doi:10.3791/58975 (2019).

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