Dergi
/
/
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

8,198 Views

07:37 min

June 11, 2019

DOI:

07:37 min
June 11, 2019

9 Views
, ,

Özet

Automatically generated

This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.

Read Article