Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Kyle  M. Loh; Amrita Palaria; Lay Teng Ang

doi:10.3791/58975

ジャーナル

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発生生物学

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Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

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発生生物学

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JoVE Journal 発生生物学

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

8,212 Views

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07:37 min

•

June 11, 2019

DOI:

10.3791/58975-v

DOI:

10.3791/58975-v

•

07:37 min

June 11, 2019

•

9 Views

Kyle M. Loh^1,2, Amrita Palaria¹, Lay Teng Ang¹

¹Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, ²Department of Developmental Biology,Stanford University School of Medicine

概要

This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.

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Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

概要

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