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Biologia do Desenvolvimento
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Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

8,206 Views

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07:37 min

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June 11, 2019

DOI:

10.3791/58975-v

DOI:
10.3791/58975-v

•

07:37 min
June 11, 2019

•

9 Views
, ,
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, 2Department of Developmental Biology,Stanford University School of Medicine

Summary

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This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.

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Cite this Article

Loh, K. M., Palaria, A., Ang, L. T. Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells. J. Vis. Exp. (148), e58975, doi:10.3791/58975 (2019).

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