Journal
/
Biología del desarrollo
/
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

8,206 Views

•

07:37 min

•

June 11, 2019

DOI:

10.3791/58975-v

DOI:
10.3791/58975-v

•

07:37 min
June 11, 2019

•

9 Views
, ,
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, 2Department of Developmental Biology,Stanford University School of Medicine

Summary

Automatically generated

This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.

Explore More Videos

Human Pluripotent Stem CellsLiver CellsDifferentiationRegenerative MedicineLiver Bud ProgenitorsHepatocyte-like CellsDevelopmental Signaling PathwaysHigh PurityIMDMPVACDM2CDM3CDM4CDM5Small MoleculesGrowth FactorsMatrixHPSCDissociation Agent

Read Article

Cite this Article

Loh, K. M., Palaria, A., Ang, L. T. Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells. J. Vis. Exp. (148), e58975, doi:10.3791/58975 (2019).

Download .ris file

Copy

Share Video

.

Copy