ジャーナル
/
免疫学と感染
/
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

7,734 Views

•

07:34 min

•

April 16, 2019

DOI:

10.3791/58829-v

DOI:
10.3791/58829-v

•

07:34 min
April 16, 2019

•

2 Views
, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

概要

Automatically generated

This protocol describes a simple method of isolating single, infected-bladder epithelial cells from a murine model of urinary tract infection.

Explore More Videos

Intracellular Bacterial CommunitiesIBCsUrinary Tract InfectionUTIMouse BladderMouth MicropipettingGlass Capillary TubeBladder Epithelial CellsSingle-cell Analysis

Read Article

Cite this Article

Yang, E., Chee, J. L., Duraiswamy, S., Chen, S., Lees, K., Chen, S. L. Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis. J. Vis. Exp. (146), e58829, doi:10.3791/58829 (2019).

Download .ris file

Copy

Share Video

.

Copy