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低酸素環境下におけるヒトのマクロファージ偏光のプロテオーム解析
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
DOI:

10:15 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:40Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Leukoreduction System Chamber (LRSC)
  • 02:34Magnetic Labeling and Isolation of CD14+ Cells (Monocytes)
  • 04:32Lysis and In-Gel Digestion
  • 07:54Results: Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
  • 09:17Conclusion

Summary

Automatic Translation

人間の大食細胞のプロテオーム署名を取得し、これをマクロファージ偏波に低酸素環境の影響の定量に適用するためのプロトコルを提案します。

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