We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization.
Court, M., Malier, M., Millet, A. Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment. J. Vis. Exp. (143), e58727, doi:10.3791/58727 (2019).