Journal
/
Bioquímica
/
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

7,812 Views

•

10:15 min

•

January 07, 2019

DOI:

10.3791/58727-v

DOI:
10.3791/58727-v

•

10:15 min
January 07, 2019

•

2 Views
, ,
1Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, 2Université Grenoble Alpes, 3Pôle Recherche,Centre Hospitalier Universitaire des Alpes

Summary

Automatically generated

We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization.

Explore More Videos

Proteomic AnalysisHuman Macrophage PolarizationLow Oxygen EnvironmentLabel-free QuantificationPBMC IsolationCD14+ Monocyte IsolationMacrophage Differentiation

Read Article

Cite this Article

Court, M., Malier, M., Millet, A. Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment. J. Vis. Exp. (143), e58727, doi:10.3791/58727 (2019).

Download .ris file

Copy

Share Video

.

Copy