Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Magali Court; Marie Malier; Arnaud Millet

doi:10.3791/58727

Journal

/

Bioquímica

/

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

JoVE Journal
Bioquímica

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Bioquímica

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

7,812 Views

•

10:15 min

•

January 07, 2019

DOI:

10.3791/58727-v

DOI:

10.3791/58727-v

•

10:15 min

January 07, 2019

•

2 Views

Magali Court^1,2, Marie Malier^1,2, Arnaud Millet^1,2,3

¹Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, ²Université Grenoble Alpes, ³Pôle Recherche,Centre Hospitalier Universitaire des Alpes

Summary

We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization.

Explore More Videos

Proteomic Analysis Human Macrophage Polarization Low Oxygen Environment Label-free Quantification PBMC Isolation CD14+ Monocyte Isolation Macrophage Differentiation

Read Article

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Summary

Automatically generated

Explore More Videos