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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Biochemistry
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JoVE Journal Biochemistry
Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

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8,314 Views

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08:31 min

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October 03, 2018

DOI:

10.3791/58307-v

DOI:
10.3791/58307-v

•

08:31 min
October 03, 2018

•

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1Department of Chemistry,Wesleyan University

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Uchendu, S. N., Rafalowski, A., Cohn, E. F., Davoren, L. W., Taylor, E. A. Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition. J. Vis. Exp. (140), e58307, doi:10.3791/58307 (2018).

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