Journal
/
Biochemistry
/
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

English

Automatically Generated

19,198 Views

•

09:02 min

•

April 20, 2018

DOI:

10.3791/57479-v

DOI:
10.3791/57479-v

•

09:02 min
April 20, 2018

•

19 Views
,
1Cluster of excellence CellNetworks,Heidelberg University, 2Heidelberg University Biochemistry Center (BZH)

Summary

Automatically generated

We provide a step-by-step protocol for split-BioID, a protein fragments-complementation assay based on the proximity-labeling technique BioID. Activated on the interaction of two given proteins, it allows the proteomics analysis of context-dependent protein complexes in their native cellular environment. The method is simple, cost-effective and only requires standard laboratory equipment.

Explore More Videos

Split-BioIDProteomic AnalysisProtein ComplexesNative Cellular EnvironmentProtein Fragment Complementation AssayProximity-dependent Protein BiotinylationCell BiologyMass Spectrometric AnalysisStreptavidin PulldownProtein Complexes RemodelingContext-specific Protein Complexes

Read Article

Cite this Article

Schopp, I. M., Béthune, J. Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment. J. Vis. Exp. (134), e57479, doi:10.3791/57479 (2018).

Download .ris file

Copy

Share Video

.

Copy