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Dize birleştirme gRNA için özelleştirilebilir bir protokol klonlama (STAgR)
JoVE Journal
Genetics
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JoVE Journal Genetics
A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
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Dize birleştirme gRNA için özelleştirilebilir bir protokol klonlama (STAgR)

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9,350 Views

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10:00 min

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December 26, 2018

DOI:

10.3791/58556-v

DOI:
10.3791/58556-v

•

10:00 min
December 26, 2018

•

4 Views
, , , , ,
1MCN Junior Research Group, Munich Center for Neurosciences,Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum,German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum,German Research Center for Environmental Health, 4Physiological Genomics,Ludwig Maximilian Universitat, BioMedical Center

Summary

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Burada, dize derleme gRNA (STAgR), klonlama mevcut CRISPR/Cas9 yaklaşıyor kolayca gRNA vektörler multiplex için bir yöntem. STAgR basit, etkili ve özelleştirilebilir gRNA çoğullama yapar.

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Cite this Article

Breunig, C. T., Neuner, A. M., Giehrl-Schwab, J., Wurst, W., Götz, M., Stricker, S. H. A Customizable Protocol for String Assembly gRNA Cloning (STAgR). J. Vis. Exp. (142), e58556, doi:10.3791/58556 (2018).

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